Tryptophan is an essential amino acid - it must be supplied externally as the body is unable to produce it on its own. It is an essential component for building proteins, hormones and neurotransmitters. Some studies suggest that it is a precursor of serotonin, a neurotransmitter linked to mental wellbeing [1]. It is possible that tryptophan deficiencies may be associated with reduced mental wellbeing following stimulant use [2], and tryptophan supplementation may have an impact on mental mood [3]. There are suggestions that tryptophan supplementation may increase tryptophan activity in the body compared to dietary tryptophan, as it may reduce competition with other LNAA amino acids that may carry a pronounced tryptophan effect [19]. This is compounded by the relatively low intake of tryptophan in the average person's diet, which is estimated to be around 1 gram per day averaged [20].Properties:Tryptophan is a precursor of serotonin, which is a neurotransmitter often associated with mood regulation and emotional control. Some sources suggest that serotonin deficiencies may be associated with the side effects of substances such as MDMA [4]. It is possible that tryptophan is associated with sleep, as it is a precursor to melatonin, a hormone that plays a role in regulating the sleep cycle [5] - the quality of which can be reduced by both the shift of bedtime relative to nighttime [6], as well as the use of many recreational drugs, such as MDMA [7]. Some studies suggest that MDMA use may affect serotonin metabolism, including by decreasing serotonin stores, decreasing serotonin receptor responses and decreasing tryptophan hydroxylase enzyme activity [8, 9, 10, 11]. Tryptophan supplementation may show beneficial effects on the psychological well-being of individuals who have experienced drug-related side effects [12, 13]. Some sources suggest that frequent MDMA use may affect tryptophan metabolism, and avoidance of this substance is recommended as a preventative measure [14]. There are suggestions that due to the pharmacokinetics of MDMA, which has a half-life of 8-9 hours, there may be a potential risk of serotonin syndrome during the period of MDMA activity in the body (equal to the five half-life of the compound). In After-Rave, tryptophan was used as a precursor of serotonin, which, unlike 5-hydroxytryptophan (5-HTP), does not bypass the enzyme tryptophan hydroxylase [16], providing a protective buffer against excessive serotonin concentrations that can lead to the health-threatening - and in some cases life-threatening - serotonin syndrome. According to some sources, the risk of serotonin syndrome as a result of tryptophan use is described as very unlikely to occur [17], whereas in the context of 5-HTP use - such a risk may be real [18], even more so in the face of circulating MDMA in the body.

List of scientific sources (to find the study easily, paste it into Google):

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Acetyl-L-carnitine (ALCAR) is a molecule that is a combination of carnitine and an acetyl group [1]. Carnitine can regulate fatty acid metabolism in the body, while the acetyl group is used in many areas, including being a component for the construction of acetylcholine [2, 3], a neurotransmitter responsible for, among other things, memory consolidation or the ability to maintain attention. This makes it all the more easy for ALCAR to replenish the pool of this neurotransmitter, thanks to its structure, which may allow it to easily penetrate the blood-brain barrier [2]. Some studies suggest that ALCAR may have a number of health-promoting properties, influencing metabolic health, the regularity of the cell's energy processes, or supporting cognitive function [4].Properties:Some studies suggest that ALCAR may exhibit a broad spectrum of neuroprotective effects, including by supporting aerobic metabolism, promoting blood circulation in the brain, antagonising the excitotoxic effects of glutamate and thus may prevent neuronal death [5]. When exposed to MDMA, ALCAR can exert effective neuroprotective effects against MDMA-induced neurotoxicity (in pre and postadministration against MDMA), at the cellular level it can reduce mitochondrial DNA deletion, improve respiratory chain expression and prevent the reduction of serotonin activity in several brain areas [6]. Carnitine itself, by promoting fatty acid transport, may enhance the properties of the neuronal mitochondrial membrane, protecting it from oxidative stress (and consequent neuronal damage) [6], which can occur during the use of, for example, MDMA as a result of the hyperthermia that occurs [16, 17]. Some studies suggest that ALCAR, through its beneficial effects on cellular health parameters and acetylcholine metabolism, may benefit the mental state of individuals who struggle with depression [7]. Reduced mental wellbeing is one of the common consequences of the abuse of many psychoactive substances, such as MDMA [8], as are nightmares or difficulty concentrating [9], which ALCAR can support [10, 11]. Substances, like MDMA, can lead to liver damage (hepatic encephalopathy can occur in some users) and severe liver failure [12, 13]. Some studies suggest that ALCAR may have the property of preventing hepatic encephalopathy and restoring normal organ function [14]. This is important because many psychoactive substances, such as MDMA and ethanol, are metabolised by the liver [15]. ALCAR can reduce symptoms of physical fatigue and mental fatigue [11, 18], which often occur after discontinuation of recreational drugs such as MDMA and other amphetamines and their derivatives [9, 19].List of scientific sources (to find the study easily, paste it into Google)

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CDP-choline is a molecule that is a combination of choline together with cytidine. Choline is a component for the production of the neurotransmitter acetylcholine, which is closely linked to memory and largely determines clarity of thought [1]. Some studies suggest that cytidine converts to uridine, a compound that influences synaptic membrane plasticity [2] and may contribute to the enhancement of the neurotransmission of dopamine - which influences the ability to maintain attention, the desire to act, gives satisfaction from the task completion process, and is linked to libido. Properties:Some studies suggest that cytidine, converted to uridine, can enhance dopamine activity by increasing the concentration of the transporter for dopamine DAT [3], by increasing the amount of dopamine released from a stimulated neuron [4], and by increasing the density of dopamine receptors [5]. CDP-choline can reduce decreases in dopamine secretion when neurons are affected by neurotoxins [6, 7], through a protective mechanism in the dopamine neuronal area. It may promote recovery from addictions to drugs such as cocaine [9], alcohol or marijuana smoking [9] due to its effects on the mesolimbic and mesocortical dopaminergic systems [10]. In a study using CDP-choline, a decrease in the frequency of cocaine use, a reduction in the desire to induce euphoria with cocaine and a decrease in perceived cocaine craving were observed in cocaine users [8], suggesting its properties may reduce the mechanism of cocaine dependence. CDP-choline may inhibit the reduction of the brain's grey matter volume in exposure to amphetamines [11], which is the origin of the popular party drug MDMA [18], as well as the attraction to this type of substance in regular users (as opposed to the placebo group). It can reduce errors associated with, among other things, difficulty in maintaining focus [12], indicating its property to improve the ability to maintain attention in concentration disorders. May influence the maintenance of the activity of the ATP-producing enzyme, acetylcholinesterase, promoting health at the cellular level [13]. It promotes glutathione activity [14] and reduces apoptosis [15], likely a consequence of preserving cell membrane plasticity, as shown in a study with experimentally induced stroke in rodents [16]. For THC abusers, it may improve emotional self-control, a disorder of which is a common symptom of drug use, and improves concentration in these individuals, deficits of which also co-occur with frequent substance use [17].List of scientific sources (to find the study easily, paste it into Google)

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L-theanine is an amino acid of non-protein origin. It is extracted from tea leaves (Camellia sinensis) [1]. Some studies suggest that it is a compound with a number of functions for the brain - through a calming mechanism, to improving memory, concentration abilities and many other aspects related to proper brain function.Properties:May inhibit the excitotoxic effects of glutamate on neurons [2]. Some psychoactive substances, such as MDMA [3], increase glutamate activity to a neurotoxic degree, which translates into side effects in the neuronal area. There are suggestions that it increases alpha wave activity in the brain, which is associated with relaxation [4]. It may improve memory and concentration [5], thereby to some extent offsetting the side effects of psychoactive substances that interfere with these processes. It may reduce the neurotoxic aspect of stress (including oxidative stress) [6, 7], which is endocrinally induced by psychoactive substances of the amphetamine type, such as MDMA [8]. In addition, it may have a beneficial effect on glutathione activity, which counteracts the cell-damaging aspect of oxidative stress [2]. Some studies indicate that it has a calming effect on the central nervous system [9] - without a dementing effect - thus may reduce the stimulant effects of selected psychoactive substances [10, 11] and facilitate sleep. It can reduce the harm of THC in terms of cognitive brain function [12], thereby reducing negative effects on parameters such as memory. It can support mental health by reducing symptoms of its disturbed state such as anxiety [13]. Anxiety is one of the common complications of regular psychoactive substance use [14].List of scientific sources (to find the study easily, paste it into Google):

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A unique feature of ALA is its ability to be soluble in both water and lipids, making it a compound that can penetrate - and have an effect - in all cell types and tissues of the body [3]. Human studies have shown that ALA can slow neurodegeneration in people with Alzheimer's disease by reducing inflammation [4,5,6]. Many psychoactive substances induce inflammation, e.g. through hyperthermia, excitotoxicity [7,8,9,10,11,12] - ALA may reduce the harmfulness of these processes. It shows protective potential against the neurotoxic effects of MDMA [13]. In a rodent study, ALA dosed 30 min prior to drug administration did not inhibit the hyperthermic effect itself, however, it completely prevented serotonin deficits and the response from glial tissue (responds with production of pro-inflammatory cytokines). Thus, ALA can significantly reduce the neurotoxic oxidative stress induced by MDMA supply. It also shows protective effects on neurons at the mitochondrial level when administered with highly neurotoxic agents such as chemotherapy [14].List of scientific sources (to find the study easily, paste it into Google):

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Vitamin B1 is an essential vitamin that, among other things, is involved in cellular energy processes, mainly linked to glucose metabolism [1]. Some studies suggest that its deficiency may lead to nervous system disorders, which may be compounded by the use of narcotic substances or dehydration.Properties:It is widely recognised that vitamin B1 is one of the key vitamins involved in alcohol metabolism, and its additional supply may reduce post-alcohol hangover [2] by supporting the enzyme that breaks down acetyl-aldehyde. There are suggestions that vitamin B1 may improve mental mood even at relatively small amounts [3]. Mental self-esteem is one of the key areas that can be affected by recreational drug use or sleepless nights. Deficiency of this vitamin can manifest as increased fatigue [4, 5] or a tendency to irritability [4], which can also be exacerbated by narcotic substances.List of scientific sources (to find the study easily, paste it into Google):

https://ods.od.nih.gov/factsheets/thiamin-healthprofessional/

doi: 10.1186/1471-2210-8-10

doi.org/10.1007/s002130050163

doi: 10.1016/j.ncl.2013.02.002

doi: 10.1089/acm.2013.0461

Oligomeric proanthocyanidins (OPCs), of which grape seed extract is a source, are compounds with very high antioxidant potential, and may thus have anti-inflammatory functions that can protect various types of tissue from damage [1].Properties:OPCs may exhibit antioxidant effects on brain cells, potentially reducing the progression of neurodegenerative processes [2, 3]. Some studies suggest that OPCs may have beneficial effects on brain functions responsible for maintaining attention, speech or memory - as shown in a study on healthy elderly individuals [4]. There are suggestions that OPCs may reduce the hepatotoxicity of even such potent agents as chemotherapeutic pharmaceuticals [5, 6]. The liver is responsible for metabolising most substances supplied to the body, including narcotic substances [7], which themselves can impair liver function, thereby potentiating their harmful effects on the body [8, 9]. Some studies have shown the efficacy of proanthocyanidins in protecting against alcohol-induced toxicity and oxidative damage [10, 11].List of scientific sources (to find a study easily, paste it into Google):

doi: 10.3390/antiox6030071

doi: 10.3945/an.114.007500

doi: 10.1007/s10571-009-9403-5

doi: 10.3389/fphar.2017.00776

doi: 10.1089/jmf.2011.0291

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Coenzyme Q10 is a lipophilic antioxidant produced by the body, whose highest concentration is reached in the cell mitochondria - the so-called 'energy furnaces' of cells [1]. This compound is thought to be mainly responsible for energy production and the reduction of oxidative stress, thus may protect cells from damage [2].Properties:Migraine headaches are - for those with such a tendency - one of the possible complications of drug substance abuse, such as MDMA [3]. Some sources suggest that Q10 may be an effective treatment for migraines [4, 5], and supplementation with it may reduce migraine frequency, duration and pain intensity [6]. In the context of migraines, there are suggestions that it shows synergy with L-carnitine [7], also included in the formulation.The need for coenzyme Q10 may increase significantly with oxidative stress [8], which is associated with the use of many psychoactive substances, such as cocaine, MDMA and alcohol. The lower the Q10 reserves, the greater the body's susceptibility to free oxygen radical-induced side effects may be [2, 9]. Some studies suggest that it supports bodily functions even in healthy individuals, possibly improving exercise performance [10,11,12], including through its ability to reduce damage to cell phospholipid membranes by free oxygen radicals, thus may reduce fatigue after a sleepless night and the use of stimulants with a negative profile of effects on cells. In the context of protective effects on the brain - coenzyme Q10 may exhibit protective effects on cellular mitochondria against oxidative stress [13,14,15], potentially reducing neurodegeneration [16,17,18,19]. In the context of stimulants, there are suggestions that: - may protect against neuronal damage induced by MDMA, cocaine, methamphetamine, alcohol [20,21,22,23] - may prevent cocaine-induced cardiac dysfunction [24].List of scientific sources (to find a study easily, paste it into Google):

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Vitamin B6 is a hydrophilic nutrient that has many functions in the body. It can affect the functioning of the nervous system, regulate mood and organ function [1].Properties:Vitamin B6, along with other micronutrients - folic acid, magnesium, calcium, iron - is involved in metabolic processes such as the production of 5-hydroxytryptophan and then serotonin from the amino acid L-tryptophan, via the enzyme tryptophan hydroxylase [2]. It is considered essential for this process. Deficiency of this vitamin may be associated with emotional disturbances in the dopamine, serotonin, GABA pathways, which may manifest as depression or anxiety disorders [3, 4]. Some human studies suggest a 2-fold higher risk of depression in people with pyridoxine deficiency [5]. Vitamin B6 may reduce the chances of neurological disorders due to its regulatory effect on homocysteine levels [6,7,8].List of scientific sources (to find a study easily, paste it into Google)\

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Folate can participate in the formation of the S-adenosine-methionine (SAM-e) molecule, a methyl group donor that positively influences the glutathione pathway (a very potent antioxidant), can positively influence mental wellbeing and regulate the function of all genes [6,7,8]. It has the advantage over folic acid that it can be the ultimate active form of folate, bypassing the limiter in the form of the enzyme dihydrofolate reductase (DHFR), which, in people with its dysfunction, causes unmetabolised to MTHF folic acid to lie biologically inactive in the body [9,10,11]. An additional benefit of supplementation with the 5-MTHF form is that in diagnostic tests, unlike folic acid, it does not mask vitamin B12 deficiency [9]. It can convert tryptophan to serotonin at the transition stage to the 5-hydroxytryptophan form and further at the transition stage of 5-hydroxytryptophan to serotonin [12].List of scientific sources (to find the study easily, paste it into Google):

doi: 10.1007/s10545-010-9177-4

doi: 10.1016/j.brainres.2008.07.074

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doi: 10.3945/ajcn.2010.29499

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Methylcobalamin is the coenzymatic form of vitamin B12, a micronutrient essential for life, responsible for the formation of red blood cells or nerve conduction. The correct status of this vitamin translates into wellbeing and cognitive function [1-5]. Properties: Methylcobalamin is absorbed much better than other available forms of vitamin B12, even in cases of impaired production of vitamin B12 transport proteins in the stomach and small intestine [4], where the number of genetic mutations involving such disorders is more than 300 per current day [3]. Regulates genetic function [3]. Lowers toxic homocysteine levels [4]. Cobalamin deficiency results in impaired production of white and red blood cells [4-5]. Cobalamin deficits have been linked in the literature to difficulty in decision-making [7,8,9], negative changes in personality and mental well-being [7, 8]. It is essential for the proper functioning of our memory [7,8, 10] and for regularities in nerve conduction by affecting nerve integrity [11]. It is essential for the synthesis of dopamine [12] and serotonin [13], whose metabolism is disrupted with the use of narcotic substances such as cocaine [14], methamphetamine [15], MDMA [16], THC [17].List of scientific sources (to find a study easily, paste it into Google):

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